Orbitrap LC-MS

Hybrid Orbitrap Mass Spectrometers	Thermo Scientific Orbitrap Exploris 120	Thermo Scientific Orbitrap Exploris 240	Thermo Scientific Orbitrap Exploris 480
Analyses		Targeted/non-targeted screening (MS) Unknown Identification (MS) Confirmation (MS/MS) Absolute and Relative Quantitation (MS and/or MS2)
Ideal Applications	Forensic Toxicology Clinical Research Food & Environmental Safety Testing Targeted/Semi Metabolomics Pharmaceuticals	Proteomics Protein Identification Forensic Toxicology Anti Doping Food and Environmental Emerging Contaminant Testing Metabolomics	Shotgun Proteomics Metabolomics TMT Multiplexing Protein Identification Host Cell Proteins Biopharma Label Free Quantification Clinical & Translational Research
Max Resolution (FWHM) @ m/z 200	120 000	240 000	480 000
Mass Accuracy	< 1 ppm	< 1 ppm	< 1 ppm
Dynamic Range	5 orders	5 orders	5 orders
Mass Range	m/z 40 – 3000	m/z 40 – 6000 Up to 8000 with BioPharma option	m/z 40 – 6000 Up to 8000 with BioPharma option
Scan Speed	Up to 22 Hz	Up to 22 Hz	Up to 40 Hz
Scan Functions	Full MS, Full MS/AIF, SIM, DIA, t-MS2, t-SIM/dd-MS2	Full MS, Full Ms/AIF, SIM, t-MS2, DIA, dd-MS2, Full MS/AIF NL/dd-MS2, Full MS/dd-MS2	Full MS, Full Ms/AIF, SIM, t-MS2, DIA, dd-MS2, Full MS/AIF NL/dd-MS2, Full MS/dd-MS2
Dissociation	In-source CID, HCD	In-source CID, HCD	In-source CID, HCD
Ion Sources	APCI/APPI Combination	APCI/APPI Combination FAIMS Pro interface EASY-Spray source Nanospray source	APCI/APPI Combination FAIMS Pro interface EASY-Spray source Nanospray source
Chromatography System recommendations	Vanquish Core Vanquish Flex Vanquish Duo Transcend	Vanquish Flex Vanquish Duo Vanquish Horizon Easy-nLC 1200 Ultimate 3000 nano	Vanquish Flex Vanquish Duo Vanquish Horizon Easy-nLC 1200 Ultimate 3000 nano

Tribrid Orbitrap Mass Spectrometers	Orbitrap ID-X Tribrid MS	Orbitrap Fusion Lumos Tribrid MS	Orbitrap Eclipse Tribrid MS
Analyses		MS and/or MSn Identification Confirmation Screening Profiling Target/Non Target Screening
Ideal Applications	Small Molecules characterization in metabolomics, Pharma candidates, Lipids	MultiOmics, ТМТ Protein Identification Intact/ Top-down proteomics Protein Interactions Targeted Quantification	MultiOmics, ТМТ Protein Identification, Intact/ Top-down proteomics Protein Interactions Targeted Quantification , Top-down mass spectrometry Biotherapeutics
AcquireX Data Acquisition	Yes	Yes	Yes
Max Resolution (FWHM) @ m/z 200	500 000	500 000	500 000
Optional резолюция 1 M FWHM	No	Yes	Yes
Mass Acuracy	< 1 ppm	< 1 ppm	< 1 ppm
Dynamic Range	5 orders	5 orders	5 orders
Mass Range	m/z 50 – 2000	m/z 200 – 6000 Up to 8000 with BioPharma option	m/z 200 – 6000 Up to 8000 with BioPharma option
Scan Speed	Up to 40 Hz	Up to 40 Hz	Up to 40 Hz
Scan Functions	Full MS, MSn, n= 1 do 10, Full Ms/AIF, SIM, t-MSn, DIA, dd-MS2, dd-MSn, Full MS/AIF NL/dd-MS2, Full MS/dd-MSn	Full MS, MSn, n= 1 do 10, Full Ms/AIF, SIM, t-MSn, DIA, dd-MS2, dd-MSn, Full MS/AIF NL/dd-MS2, Full MS/dd-MSn	Full MS, MSn, n= 1 do 10, Full Ms/AIF, SIM, t-MSn, DIA, dd-MS2, dd-MSn, Full MS/AIF NL/dd-MS2, Full MS/dd-MSn
Ion Sources	APCI/APPI Combination	APCI/APPI Combination FAIMS Pro interface EASY-Spray source Nanospray source	APCI/APPI Combination FAIMS Pro interface EASY-Spray source Nanospray source
Chromatography System recommendations	Vanquish Horizon Vanquish Duo Transcend	Vanquish Horizon Easy-nLC 1200 Ultimate 3000 nano	Vanquish Horizon Easy-nLC 1200 Ultimate 3000 nano